کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6240630 1280432 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Targeting bacterial integration host factor to disrupt biofilms associated with cystic fibrosis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی ریوی و تنفسی
پیش نمایش صفحه اول مقاله
Targeting bacterial integration host factor to disrupt biofilms associated with cystic fibrosis
چکیده انگلیسی

BackgroundThis study aims to identify whether the bacterial protein, Integration Host Factor (IHF), is present within sputum solids collected from cystic fibrosis (CF) patients and thus might contribute to the structural stability of biofilms within the lungs.MethodsThe presence of IHF in sputum was determined by immunohistochemistry. The role of IHF in stabilizing biofilms within sputum was tested in vitro wherein anti-IHF was used to attempt to dissolve sputum solids.ResultsThirty-seven of 44 sputum samples (84%) were positive for anti-IHF staining. Treatment with anti-IHF or DNase of 6 representative samples, dissolved sputum solids significantly better than treatment with normal saline in vitro, and strong synergism was observed when these agents were used in combination.ConclusionsIHF was detected in the majority of sputum samples from patients with CF and in vitro treatment with anti-IHF induced dissolution of sputum solids. These data support further investigation of IHF as a potential therapeutic target for patients with CF.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cystic Fibrosis - Volume 12, Issue 4, July 2013, Pages 384-389
نویسندگان
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