Simultaneous extraction and purification of aloe polysaccharides and proteins using ionic liquid based aqueous two-phase system coupled with dialysis membrane

► Aloe polysaccharides and the impurity of proteins were simultaneously studied during the ILATPS extraction. ► Aloe polysaccharides in salt-rich phase was further purified using a dialysis membrane to remove the salt and IL. ► The monosaccharide constitutes of aloe polysaccharides were analyzed by a HPLC method. ► Purity of the final product was demonstrated by TGA. ► Ionic liquid was recycled by a simple solvent extraction.