کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6247182 | 1284514 | 2015 | 6 صفحه PDF | دانلود رایگان |
- Six diagnostic methods on respiratory viral infection are explored in this study.
- Monoclonal antibodies produced with hybridoma technology are used.
- A new technique to simultaneously detect 6 kinds of febrile respiratory viruses in a protein chip was developed.
- The accuracy rate for this method is >99.65%, and is inexpensive and capable of high-precision and high-throughout screening.
Acute respiratory infection is a frequently transmitted illness of concern to doctors and patients. Considering its airborne transmission, early diagnosis of such disease is particularly important. This study explored respiratory viral infections with influenza virus, parainfluenza virus, respiratory syncytial virus, human metapneumovirus, human bocavirus, coronavirus, and other early diagnostic substances as confirmed by literature resources. This study also used the corresponding monoclonal antibodies that were produced with the use of hybridoma technology, which were fixed on the chip after purification, for further serum detection. Using this method, a new technique to simultaneously detect 6 kinds of febrile respiratory viruses in a protein chip was developed. The accuracy rate of this method can be >99.65%. This product is inexpensive and capable of high-precision and high-throughput screening, which are prominent advantages.
Journal: Transplantation Proceedings - Volume 47, Issue 6, JulyâAugust 2015, Pages 1790-1795