کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6253422 1612526 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Shock/Sepsis/Trauma/Critical CareBeneficial effects of dantrolene on sepsis-induced diaphragmatic dysfunction are associated with downregulation of high-mobility group box 1 and calpain-caspase-3 proteolytic pathway
ترجمه فارسی عنوان
شوک / سپسیس / تروما / مراقبت بحرانی اثرات مفیدی دانترولن بر اختلال عملکرد دیافراگمی ناشی از سپسیس با کاهش فرکانس گروه پروتئولیتیک کالپین-کاسپ 3
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
چکیده انگلیسی

BackgroundIntracellular calcium overload is a major contributing factor to diaphragmatic dysfunction triggered by sepsis. In this study, the possible role of dantrolene, a ryanodine receptor inhibitor, in preventing the release of calcium from the sarcoplasmic reticulum in diaphragmatic dysfunction and weakness was explored.MethodsA middle-grade severity sepsis rat model was established for the effects of treatment with dantrolene, on diaphragm harvested 24 h after cecal ligation and puncture (CLP), and analyzed using functional, histologic, and biomarker assays.ResultsIt was found that in septic rats, treatment with dantrolene significantly improved the contractility, relaxation, and fatigue index of the diaphragm in a dose-dependent manner. The benefits are associated with improvement in ultrastructural changes of Z band integrity and myofilament arrangements along with increases both in the ratio of slow-twitch type composition. Moreover, dantrolene effectively inhibits the overexpression of high-mobility group box 1 and reduces the calpain-1-caspase-3 proteolytic activity.ConclusionsDantrolene can effectively attenuate the dysfunction of diaphragm in septic rats; Furthermore, the beneficial effects were associated with downregulation of high-mobility group box 1 and calpain-1-caspase-3 proteolytic activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 200, Issue 2, February 2016, Pages 637-647
نویسندگان
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