کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6254057 1288418 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Wound Healing/Plastic SurgeryActivation of mitosis and angiogenesis in diabetes-impaired wound healing by processed human amniotic fluid
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی عمل جراحی
پیش نمایش صفحه اول مقاله
Wound Healing/Plastic SurgeryActivation of mitosis and angiogenesis in diabetes-impaired wound healing by processed human amniotic fluid
چکیده انگلیسی

BackgroundFunctional characterization of human amniotic fluid (AF) proteome, 845 proteins, has revealed that top three functions are cell proliferation, movement and differentiation, events fundamental to development, and tissue repair. Although these findings fortify the idea that AF components play roles in regeneration-like fetal wound healing, it is not known whether the components endure processing. Therefore, we processed AF and tested its effects on diabetes-impaired wound healing in an animal model.Materials and methodsThrough a germfree procedure, mature and premature AF samples were collected, respectively, from the mothers of full-term and preterm infants. Excisional wounds were generated on the dorsum of diabetic rats. Wounds were treated on day 3 and harvested on day 7 postwounding. Proliferating cell nuclear antigen and alpha-smooth muscles actin, markers for mitosis and angiogenesis, respectively, were assessed by in situ immunodetection method.ResultsSignificant increases in the rate of wound closure and proliferating cell nuclear antigen-expressing cells were observed in AF-treated wounds when compared with that of sham and control wounds. Likewise, the number of large vessels was significantly increased in the wounds treated with the AF. However, population of myofibroblasts was not affected by the treatment. The mature and premature AF were almost equally effective.ConclusionsOur data, for the first time, show that processed AF accelerates diabetes-impaired wound healing by activating mitosis and angiogenesis, indicating that bioactive molecules in AF may endure processing. We believe that processed forms of this naturally designed “Cocktail” of bioactive molecules may have multiple clinical applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Surgical Research - Volume 188, Issue 2, 15 May 2014, Pages 545-552
نویسندگان
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