کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6254145 | 1288424 | 2014 | 9 صفحه PDF | دانلود رایگان |
BackgroundMast cells (MCs) play a role in ischemia-reperfusion (I/R) injury in many organs. However, a recent study found that MCs are not involved in I/R injury in isolated rat livers that were perfused only for 1Â h. The purpose of this study is to reevaluate the role of MCs in hepatic I/R injury in rat.Materials and methodsA warm hepatic I/R injury model of 1Â h ischemia followed by 24Â h of reperfusion was used. MC modulation was induced via cromolyn injection or a method called MC depletion using compound 48/80. The effects of MC modulation were evaluated by toluidine blue staining and assessment of mast cell tryptase in sera. The role of MCs in I/R injury was evaluated by hematoxylin and eosin staining graded by Suzuki criteria, alanine aminotransferase and aspartate aminotransferase levels in sera, and malondialdehyde levels in liver homogenates.ResultsFirst, MC degranulation peaked after 2Â h of reperfusion and liver damage peaked after approximately 6Â h of reperfusion. Second, a method called MC depletion previously used in the skin with repeated injections of compound 48/80 worked similarly in the hepatic setting. Third, stabilization of MCs with cromolyn or depletion of MCs with compound 48/80 each decreased hepatic I/R injury. The most noticeable effects of cromolyn and compound 48/80 treatment were observed after approximately 6Â h of reperfusion.ConclusionsMC degranulation promotes hepatic I/R injury in rats.
Journal: Journal of Surgical Research - Volume 186, Issue 1, January 2014, Pages 170-178