کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6256177 | 1612931 | 2016 | 9 صفحه PDF | دانلود رایگان |
- Chronic ethanol vapor inhalation dose-dependently suppressed nest building for up to 32Â h after withdrawal.
- Withdrawal from an acute, high dose (4Â g/kg) of ethanol reduced nest building for up to 32Â h.
- Body temperature predicted nest building in naïve HS/Npt mice but not in the same mice following ethanol vapor chamber exposure.
Withdrawal after chronic ethanol (EtOH) affects body temperature, goal-directed behavior and motor function in mice and increases general central nervous system excitability. Nest-building tests have been used to assay these states but to this point have not been employed as measures of EtOH withdrawal severity. We first refined nest-scoring methods using a genetically heterogeneous stock of mice (HS/Npt). Mice were then made physically dependent following three days of chronic EtOH vapor inhalation to produce average blood EtOH concentrations (BECs) of 1.89Â mg/mL. EtOH withdrawal affected the progression of nest building over time when mice were tested 2-4 days after removal from three days of chronic exposure to EtOH. In a separate group of mice, chronic EtOH vapor inhalation (BECs 1.84Â mg/mL) suppressed nest building over days 1-2 but not days 2-3 of withdrawal. In a following experiment, EtOH withdrawal dose-dependently slowed recovery of nest building for up to 32Â h. Finally, we determined that long-lasting nest-building deficits extend to mice undergoing withdrawal from a high dose (4Â g/kg) of acute EtOH. Sex differences for nest building were absent following EtOH exposure. In mice naïve to EtOH treatments, male mice had lower pre-test body temperatures and increased nest scores across a two-day testing period compared to females. These results suggest that nest building can be used to assess chronic and acute EtOH withdrawal severity in mice.
Journal: Behavioural Brain Research - Volume 302, 1 April 2016, Pages 182-190