کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6256466 1612940 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chronic MPTP treatment produces hyperactivity in male mice which is not alleviated by concurrent trehalose treatment
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
Chronic MPTP treatment produces hyperactivity in male mice which is not alleviated by concurrent trehalose treatment
چکیده انگلیسی


- The MPTP + probenecid (MPTPp) paradigm is a successful model of Parkinson's disease.
- MPTPp caused significant and replicable hyperactivity in adult male mice.
- Addition of a 1% trehalose solution did not prove neuroprotective.
- Tests of olfaction, grip strength, exploration, water maze performance were also unaffected.

The chronic MPTP + probenecid treatment paradigm has been used to successfully model the neurochemical, neuropathological, and behavioral effects associated with Parkinson's disease. Here, adult male C57Bl/6 mice were injected ip with 25 mg/kg MPTP and 250 mg/kg probenecid (MPTPp) or saline twice weekly for a total of 10 injections. Behavioral assessments included motor coordination, grip strength, spatial learning/memory, locomotor activity, and anhedonia. Those assessments were repeated up to 8 weeks post-treatment. In a subsequent experiment, adult male mice were treated with saline or MPTPp as described above. One-half of each group was allowed access to 1% trehalose in the water bottle. Trehalose intake averaged 1.90-2.34 g/kg. Behavioral assessments included locomotor activity, olfaction, motor coordination, grip strength, and exploratory behavior. Those assessments were repeated 4 weeks post-treatment. The strongest MPTPp effect was hyperactivity as exhibited in the open field. This increased activity was apparent in both experiments and occurred at all time points post-treatment. Assessments of grip strength, water maze performance, olfaction, and exploratory behavior did not indicate MPTPp-related alterations. When the specifications for the motor coordination test were made somewhat easier in the second experiment, there were deficits exhibited by the MPTPp group, the MPTPp + trehalose group and the trehalose group. The addition of trehalose did not alleviate any of the MPTPp-induced behavioral alterations; however, trehalose treatment significantly attenuated the striatal decreases in DA, DOPAC, HVA and 5-HIAA. These results provide a more comprehensive description of the behavioral alterations resulting from the chronic MPTPp treatment regimen and suggest that trehalose at this concentration does not act as a complete neuroprotectant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research - Volume 292, 1 October 2015, Pages 68-78
نویسندگان
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