کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6262023 1290788 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportNeuroprotective effect of early and short-time applying sophoridine in pMCAO rat brain: Down-regulated TRAF6 and up-regulated p-ERK1/2 expression, ameliorated brain infaction and edema
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Research reportNeuroprotective effect of early and short-time applying sophoridine in pMCAO rat brain: Down-regulated TRAF6 and up-regulated p-ERK1/2 expression, ameliorated brain infaction and edema
چکیده انگلیسی

BackgroundMatrine has been proven to protect ischemic injury in brain and sophoridine (SOP) is an isomeride of matrine. It is unknown whether SOP has this protective effect on ischemic injury in brain. We therefore investigated the potential neuroprotective role of SOP and the underlying mechanism.MethodsMale, Sprague-Dawley rats were randomly assigned into five groups: Vehicle (pMCAO + saline), High dose (pMCAO + SOP 10 mg/kg), Middle dose (pMCAO + SOP 5 mg/kg), Low dose (pMCAO + SOP 2.5 mg/kg) and Sham operated group. Permanent middle cerebral artery occlusion (pMCAO) model was used and SOP was administered intraperitoneally immediately after cerebral ischemia and once daily in the following days. Neurological deficit was evaluated using a modified six point scale; brain water content and infarct volume were measured. The expression of TRAF6 and ERK1/2 were measured by immunohistochemistry, Western blotting.ResultsCompared with Vehicle group, the cerebral edema was alleviated in High dose group (P < 0.05), and the infarct volume was decreased in Low dose group (P < 0.05). Consistent with these results, immunohistochemistry and Western blot analysis indicated that TRAF6 expression was significantly decreased in SOP administrated groups at 24 h, and the expression of phosphorylated ERK1/2 increased in Low dose at 72 h.ConclusionsSOP protected the brain from damage caused by pMCAO, and this effect may be through down-regulation of TRAF6 expression and up-regulation of ERK1/2 phosphorylation expression.

► Rat middle cerebral artery occlusion model is used to analyse sophoridine's effect. ► Sophoridine ameliorates brain edema and infarct size. ► Sophoridine up-regulates expression of ERK1/2 phosphorylation and down-regulates TRAF6. ► Sophoridine is neuroprotective in the acute phase of cerebral ischemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 88, Issue 4, 1 July 2012, Pages 379-384
نویسندگان
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