کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6262058 | 1613272 | 2011 | 5 صفحه PDF | دانلود رایگان |
This study presents our findings on the extent of neuronal damage in the hippocampal CA1-4 subfields following global (forebrain) cerebral ischemia in rats when using different blood pressure levels (37 vs 45Â mmHg) and bilateral carotid occlusion durations (8 vs 10Â min) under isoflurane anesthesia. We observed that global ischemia induced at a blood pressure of 37Â mmHg resulted in high-grade CA1 neuron injury (>90%) at either duration of carotid occlusion. In contrast, global ischemia induced at a blood pressure of 45Â mmHg resulted in either high-grade CA1 neuronal loss or a neuronal loss of â50% or less. We also noted that a post-reperfusion EEG recovery time (return of burst suppression spikes) of >12Â min was associated with an 85% rate of high-grade CA1 neuronal injury. Neuronal loss in the other hippocampal subfields did not differ significantly between any of the 4 different model parameters tested. In these subfields â55% neuronal loss occurred in the CA2 subfield, and â30% in the CA3 and CA4 subfields. These findings highlight the need to assess different model parameters in order to achieve consistent high-grade CA1 neuronal damage, which, among other experimental outcomes, will improve the ability to uncover therapeutic effects using the least possible animals when assessing a neuroprotective treatment.
⺠37 mmHg and 8 or 10 min of ischemia resulted in high grade CA 1 neuronal loss. ⺠45 mmHg and 8 or 10 min of ischemia resulted in inconsistent CA 1 neuronal loss. ⺠Long EEG recovery times (>12 min) were associated with high-grade CA 1 neuronal loss.
Journal: Brain Research Bulletin - Volume 86, Issues 5â6, 25 November 2011, Pages 390-394