کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6262345 1292351 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportNeuroprotection comparison of chlorogenic acid and its metabolites against mechanistically distinct cell death-inducing agents in cultured cerebellar granule neurons
ترجمه فارسی عنوان
گزارش تحقیقاتی مقایسه نانوذرات اسید کلروژنیک و متابولیت های آن در برابر عوامل مؤثر در مرگ و میر ناشی از مکانیکی سلول های بنیادی در گره های مغزی کشتار شده
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Chlorogenic acid (CGA) and its metabolites are major polyphenols found in coffee.
- CGA and caffeic acid protected cerebellar granule neurons (CGNs) from nitric oxide.
- Caffeic acid and ferulic acid protected CGNs from glutamate excitotoxicity.
- Caffeic acid uniquely rescued CGNs from a variety of other stressors.
- Caffeic acid displays a much broader neuroprotective profile than CGA.

While the number of patients diagnosed with neurodegenerative disorders like Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease is increasing, there are currently no effective treatments that significantly limit the neuronal cell death underlying these diseases. Chlorogenic acid (CGA), a polyphenolic compound found in high concentration in coffee, is known to possess antioxidant and free radical scavenging activity. In this study, we investigated the neuroprotective effects of CGA and its major metabolites in primary cultures of rat cerebellar granule neurons. We show that CGA and caffeic acid displayed a dramatic protective effect against the nitric oxide donor, sodium nitroprusside. In marked contrast, ferulic acid and quinic acid had no protective effect against this nitrosative stress. While CGA and quinic acid had no protective effect against glutamate-induced cell death, caffeic acid and ferulic acid significantly protected neurons from excitotoxicity. Finally, caffeic acid was the only compound to display significant protective activity against hydrogen peroxide, proteasome inhibition, caspase-dependent intrinsic apoptosis, and endoplasmic reticulum stress. These results indicate that caffeic acid displays a much broader profile of neuroprotection against a diverse range of stressors than its parent polyphenol, CGA, or the other major metabolites, ferulic acid and quinic acid. We conclude that caffeic acid is a promising candidate for testing in pre-clinical models of neurodegeneration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1648, Part A, 1 October 2016, Pages 69-80
نویسندگان
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