کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6262386 1613796 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportSustained alterations in neuroimmune gene expression after daily, but not intermittent, alcohol exposure
ترجمه فارسی عنوان
گزارش تحقیق تغییرات در بیان ژن نورونی پس از روزانه، اما نه متناوب، در معرض الکل قرار دارد
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Alcohol significantly increased IL-6 and Iκβα and reduced IL-1β and TNFα in the CNS.
- Increased IL-6 and IκBα persisted after 6 daily but not 6 intermittent EtOH exposures.
- Mild metabolic tolerance was observed after intermittent but not daily EtOH exposure.
- Neuroimmune consequences of EtOH were contingent upon schedule of EtOH delivery.
- These data shed important light on intoxication-related changes in neuroimmune genes.

Acute ethanol intoxication is associated with Rapid Alterations in Neuroimmune Gene Expression (RANGE), including increased Interleukin (IL)-6 and Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα), and suppressed IL-1β and Tumor necrosis factor (TNF) α, yet little is known about adaptations in cytokines across the first few ethanol exposures. Thus, the present studies examined central cytokines during intoxication (3 h post-ethanol) following 2, 4 or 6 intragastric ethanol challenges (4 g/kg) delivered either daily or every-other-day (EOD). Subsequent analyses of blood ethanol concentrations (BECs) and corticosterone were performed to determine whether the schedule of ethanol delivery would alter the pharmacokinetics of, or general sensitivity to, subacute ethanol exposure. As expected, ethanol led to robust increases in IL-6 and IκBα gene expression in hippocampus, amygdala and bed nucleus of the stria terminalis (BNST), whereas IL-1β and TNFα were suppressed, thereby replicating our prior work. Ethanol-dependent increases in IL-6 and IκBα remained significant in all structures - even after 6 days of ethanol. When these doses were administered EOD, modest IL-6 increases in BNST were observed, with TNFα and IL-1β suppressed exclusively in the hippocampus. Analysis of BECs revealed a small but significant reduction in ethanol after 4 EOD exposures - an effect which was not observed when ethanol was delivered after 6 daily intubations. These findings suggest that ethanol-induced RANGE effects are not simply a function of ethanol load per se, and underscore the critical role that ethanol dosing interval plays in determining the neuroimmune consequences of alcohol.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1646, 1 September 2016, Pages 62-72
نویسندگان
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