کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6262435 1613796 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportProbing amyloid beta-induced cell death using a fluorescence-peptide conjugate in Alzheimer's disease mouse model
ترجمه فارسی عنوان
گزارش تحقیق در مورد مرگ سلولی ناشی از بتا آمیلوئید با استفاده از یک اتصال دهنده پپتید فلورسانس در مدل موش های آلزایمر
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Apoptosis caused by Aβ has been shown to play an important role in AD pathology.
- The ApoPep-1 co-localized with brain cells which undergo apoptosis in AD mice.
- ApoPep-1 could be an assistant tool for molecular imaging in AD diagnosis.

With the increasing worldwide incidence of Alzheimer's disease (AD), there is a critical need for the discovery of more effective diagnostic methods. However, development of diagnostic tools in AD has been hindered by obstacles such as the absence of exact biomarkers. Apoptosis caused by amyloid-β (Aβ) plays an important role in AD pathology; therefore, provides an attractive biological target for the diagnosis of AD. The present study aimed to evaluate the potential of small peptide, named ApoPep-1 (Apoptosis-targeting peptide-1) as a new apoptosis imaging agent in AD. The fluorescein-conjugated ApoPep-1, but not the control peptide, targeted apoptotic cells in the brain of amyloid precursor protein (APP)/presenilin 1 (PS1) mice. We also observed fluorescence signals during in vivo imaging of apoptotic cells using ApoPep-1, and fluorescence levels increased in an age-dependent manner in APP/PS1 mice. Ex vivo imaging of isolated brains in APP/PS1 mice further confirmed the targeting of ApoPep-1 to apoptotic cells. The fluorescein-labeled ApoPep-1 co-localized with brain cells such as neurons, astrocytes, and microglia, all of which undergo apoptosis in the APP/PS1 mice brain. These findings demonstrate that ApoPep-1 can target apoptotic brain cells, and be used for experimental investigations relevant to apoptosis in AD.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1646, 1 September 2016, Pages 514-521
نویسندگان
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