کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6262565 1613804 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportFlow cytometric characterization of microglia in the offspring of PolyI:C treated mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research ReportFlow cytometric characterization of microglia in the offspring of PolyI:C treated mice
چکیده انگلیسی


- Iba1 is a reliable antibody to analyze its expression via flow cytrometry.
- Iba1 expression in the PolyI:C model indicates changes in microglial activation.
- Differences in expression of CD11b, CD45 and Iba1 are age and gender-specific.
- PolyI:C leads to age and gender specific alterations in CD11b and CD45 expression.

The neuropathology of schizophrenia has been reported to be closely associated with microglial activation. In a previous study, using the prenatal PolyI:C schizophrenia animal model, we showed an increase in cell numbers and a reduction in microglial branching in 30-day-old PolyI:C descendants, which suggests that there is microglial activation during adolescence. To provide more information about the activation state, we aimed to examine the expression levels of Iba1, which was reported to be up-regulated in activated microglia. We used a flow cytometric approach and investigated CD11b and CD45, two additional markers for the identification of microglial cells. We demonstrated that intracellular staining against Iba1 can be used as a reliable flow cytometric method for identification of microglial cells. Prenatal PolyI:C treatment had long-term effects on CD11b and CD45 expression. It also resulted in a trend towards increased Iba1 expression. Imbalance in CD11b, CD45, and Iba1 expression might contribute to impaired synaptic surveillance and enhanced activation/inflammatory activity of microglia in adult offspring.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1636, 1 April 2016, Pages 172-182
نویسندگان
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