کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263004 1613823 2015 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportCochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments
ترجمه فارسی عنوان
مطالعات تحقیقاتی اکسپلورهای کلاسیک هسته ای کچلر، تمایز سلول های بنیادی عصبی از هسته حلزونی را در آزمایش های همکاری فرهنگ ترویج می دهند
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- The cochlear nucleus (CN) encompasses an environment for the differentiation of NSCs.
- NSCs from the CN are capable to differentiate after engraftment in brain tissue.
- NSCs from the hippocampus can engraft after co-cultivation in the cochlear nucleus.
- Laminin is up-regulated in CN whole-mount explants and does regulate the differentiation of cochlear nucleus NSCs.
- Activation of NSCs in the CN might applicable in the therapy of hearing loss.

The cochlear nucleus is the first brainstem nucleus to receive sensory input from the cochlea. Depriving this nucleus of auditory input leads to cellular and molecular disorganization which may potentially be counteracted by the activation or application of stem cells. Neuronal stem cells (NSCs) have recently been identified in the neonatal cochlear nucleus and a persistent neurogenic niche was demonstrated in this brainstem nucleus until adulthood. The present work investigates whether the neurogenic environment of the cochlear nucleus can promote the survival of engrafted NSCs and whether cochlear nucleus-derived NSCs can differentiate into neurons and glia in brain tissue. Therefore, cochlear nucleus whole-mount explants were co-cultured with NSCs extracted from either the cochlear nucleus or the hippocampus and compared to a second environment using whole-mount explants from the hippocampus. Factors that are known to induce neuronal differentiation were also investigated in these NSC-explant experiments. NSCs derived from the cochlear nucleus engrafted in the brain tissue and differentiated into all cells of the neuronal lineage. Hippocampal NSCs also immigrated in cochlear nucleus explants and differentiated into neurons, astrocytes and oligodendrocytes. Laminin expression was up-regulated in the cochlear nucleus whole-mounts and regulated the in vitro differentiation of NSCs from the cochlear nucleus. These experiments confirm a neurogenic environment in the cochlear nucleus and the capacity of cochlear nucleus-derived NSCs to differentiate into neurons and glia. Consequently, the presented results provide a first step for the possible application of stem cells to repair the disorganization of the cochlear nucleus, which occurs after hearing loss.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1616, 7 August 2015, Pages 58-70
نویسندگان
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