کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
626313 1455445 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Integrated inline filtration: A method to produce highly concentrated retroviral vector titer supernatant
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Integrated inline filtration: A method to produce highly concentrated retroviral vector titer supernatant
چکیده انگلیسی

Pseudotype vectors are promising for gene transfer in many gene therapy approaches, however low vector concentration in batch cultures and high temperature-dependent decay limit sufficiently large-scale production. The ability of commercial ceramic asymmetric ultrafiltration membranes with a cut-off of 20 kDa to purify retroviral pseudotype vectors derived from the murine leukemia virus (MLV) carrying the HIV-1 envelope protein MLV (HIV-1) was studied.Experimental study was carried out in order to analyse the impact of inline tangential flow filtration (TFF) combined with different harvest temperatures in continuous production mode compared to batch filtration. Retroviral pseudotype vectors were produced using a 200 ml fixed bed reactor for high cell density cultivation on macro porous carriers up to 400 hours. By tangential flow ultrafiltration combined with cooling down the harvest supernatant to 4°C, the vector concentration was increased 12-fold with an average recovery of 78.7% of the initial infective capacity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Desalination - Volume 245, Issues 1–3, 15 September 2009, Pages 614-620