کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6263621 1613904 2013 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportCalmodulin-dependent regulation of neurotransmitter release differs in subsets of neuronal cells
ترجمه فارسی عنوان
گزارش تحقیق گزارشات کالدولولین وابسته به انتشار نورواترنمایان در زیر سلول های سلول های عصبی متفاوت است
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Ophiobolin A (OBA) is a potent calmodulin inhibitor.
- OBA suppressed neurotransmitter release from only a subset of neuronal cells.
- OBA suppressed Ca2+-induced dissociation of myosin Va from secretory vesicles.
- Myosin Va was associated with secretory vesicles in a subset of neuronal cells.
- Myosin Va may be a major target of OBA in the suppression of neurotransmitter release.

The purpose of this study was to determine whether calmodulin (CaM) plays a role in neurotransmitter release by examining the effect that ophiobolin A (OBA), a CaM antagonist, on neurotransmitter release from clonal rat pheochromocytoma PC12 cells, primary cortical neurons, and primary cerebellar granule cells. OBA inhibited Ca2+/CaM-dependent phosphorylation of cAMP response element binding protein in all cell types tested. Moreover, Ca2+-dependent release of dopamine and acetylcholine from PC12 cells were remarkably reduced by OBA in a dose-dependent and temporal manner, but neurotransmitter release partially recovered with the addition of CaM in membrane permeabilized PC12 cells. OBA and several synthetic CaM antagonists suppressed Ca2+-dependent glutamate release from cerebral cortical neurons, but not from cerebellar granule cells. Myosin Va, a CaM binding protein, localized to synaptic vesicles of PC12 cells and cerebral cortical neurons, but not in cerebellar granule cells. OBA suppressed Ca2+-induced myosin Va dissociation from secretory vesicles, and inhibited secretory vesicle motility in PC12 cells. These results suggest that CaM, although not essential, regulates neurotransmitter release in a subset of neurons and secretory cells, and myosin Va is a possible target of OBA in this process.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1535, 16 October 2013, Pages 1-13
نویسندگان
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