کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6264383 1613987 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportEffects of microinjections of Group II metabotropic glutamate agents into the amygdala on sleep
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research ReportEffects of microinjections of Group II metabotropic glutamate agents into the amygdala on sleep
چکیده انگلیسی

Systemic administration of the Group II metabotropic glutamate (mGlu) receptor agonist, LY379268 (LY37), dose-dependently suppresses rapid eye movement sleep (REM) whereas systemic administration of the mGlu II receptor antagonist, LY341495 (LY34), increases arousal. Group II mGlu receptors are highly expressed in the amygdala, a brain region involved in the regulation of sleep and arousal. To determine whether the amygdala is involved in mediating the effects of Group II mGlu agents on sleep, we microinjected LY37 and LY34 into the basal amygdala (BA) and the central nucleus of the amygdala (CNA) and recorded sleep and wakefulness. Wistar rats were implanted with electrodes for recording sleep and with bilateral cannulae aimed into BA for drug administration. Different groups of rats received bilateral microinjections of LY37 into BA at two dosage ranges (3.2 mM, 5.3 mM or 10.7 mM or 0.1 nM, 2.0 nM or 10.0 nM) or one dosage range of LY34 (1.0 nM, 30.0 nM or 60.0 nM). Microinjections into CNA were conducted at one dosage range for LY37 (0.1 nM, 2.0 nM or 10.0 nM) and for LY34 (1.0 nM, 30.0 nM or 60.0 nM). All drugs or vehicle alone were administered in a counterbalanced order at 5-day intervals. Following microinjection, sleep was recorded for 20 h. Microinjection of LY37 into BA at both nM and mM concentrations significantly decreased REM without significantly altering NREM, total sleep or wakefulness. The high dosage of LY34 in BA significantly suppressed NREM and total sleep. Microinjections of LY37 or LY34 into CNA had no significant impact on sleep. We suggest that Group II mGlu receptors may influence specific cells in BA that control descending output (via the CNA or bed nucleus of the stria terminalis) that in turn regulates pontine REM generator regions.

► We tested the effects of Group II metabotropic (mGlu II) agents in the amygdala on sleep. ► LY379268, mGlu II agonist, injected into basal amygdala decreased rapid eye movement sleep. ► LY341495, mGlu II antagonist, injected into basal amygdala decreased sleep. ► Neither drug injected into central nucleus of the amygdala impacted sleep. ► The basal amygdala is a site of action for the effects of mGlu II agents on sleep.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1452, 3 May 2012, Pages 85-95
نویسندگان
, , , ,