کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6265221 1614066 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportIncreased susceptibility to ischemia and macrophage activation in STZ-diabetic rat nerve
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research ReportIncreased susceptibility to ischemia and macrophage activation in STZ-diabetic rat nerve
چکیده انگلیسی

Ischemic vulnerability in diabetic nerve plays a paramount role in the development of diabetic neuropathy, yet little is known of the underlying mechanism. Diabetes enhances the inflammatory response to ischemia and reperfusion. We investigated pathological characteristics of nerve fibers and endoneurial macrophages along the length of sciatic-tibial nerves before and after ischemia (60 to 90 min) and reperfusion (6 h to 7 days) in 8 weeks of STZ-induced diabetic rats. Without ischemia, diabetic nerves revealed significantly increased the density of Iba-1-positive endoneurial macrophages when compared with controls. Most of macrophages appeared slim and triangular in shape, but in diabetic nerves, some were rounded with bromodeoxyuridine (BrdU) incorporation, suggesting proliferating macrophages. Seventy-five minutes of ischemia is the minimal ischemic time to cause pathological changes in diabetic nerves. Following 90 min of ischemia and 6 h of reperfusion in diabetic rats, the number of Iba-1-positive endoneurial macrophages was increased significantly at the thigh level of sciatic nerve when compared with those before ischemia. Endoneurial macrophages in diabetic nerves increased in number further significantly after 24 and 48 h of reperfusion and underwent morphological alterations; swollen and rounded including phagocytosis. After 90 min of ischemia and 7 days of reperfusion, severe pathological alterations, e.g., demyelination and endoneurial edema at proximal nerves and axonal degeneration distally, were observed in diabetic nerves, while control nerves showed normal morphology. We conclude that macrophage proliferation occurs in STZ-diabetic nerves. The acute inflammatory response after ischemia and reperfusion was intensified in diabetic nerves. Activation of resident macrophages and infiltration by recruited macrophages could be casually linked to ischemic susceptibility in diabetic nerve.

Research Highlights► Diabetic rat nerve shows increased susceptibility to ischemia and reperfusion. ► Seventy-five minutes of ischemia causes pathological changes in diabetic nerves. ► Macrophages including proliferating macrophages were increased in diabetic nerve. ► Diabetic nerve enhances inflammatory response to reperfusion injury. ► Macrophage activation was linked to ischemic susceptibility in diabetic nerve.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1373, 10 February 2011, Pages 172-182
نویسندگان
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