Basic NeuroscienceApplication of 3-D imaging sensor for tracking minipigs in the open field test

- EthoStudio and 3-D sensor were applied for automated tracking of minpig behavior.
- 3-D sensor can trace white, black and agouti minipigs in the open field test.
- Agouti minipigs explored arena more than white or black animals.
- 3-D imaging system can be used to study behavior of farm-bred animals.

BackgroundThe minipig is a promising model in neurobiology and psychopharmacology. However, automated tracking of minipig behavior is still unresolved problem.New methodThe study was carried out on white, agouti and black (or spotted) minipiglets (n = 108) bred in the Institute of Cytology and Genetics. New method of automated tracking of minipig behavior is based on Microsoft Kinect 3-D image sensor and the 3-D image reconstruction with EthoStudio software. The algorithms of distance run and time in the center evaluation were adapted for 3-D image data and new algorithm of vertical activity quantification was developed.ResultsThe 3-D imaging system successfully detects white, black, spotted and agouti pigs in the open field test (OFT). No effect of sex or color on horizontal (distance run), vertical activities and time in the center was shown. Agouti pigs explored the arena more intensive than white or black animals, respectively. The OFT behavioral traits were compared with the fear reaction to experimenter. Time in the center of the OFT was positively correlated with fear reaction rank (ρ = 0.21, p < 0.05). Black pigs were significantly more fearful compared with white or agouti animals.Comparison with existing methodThe 3-D imaging system has three advantages over existing automated tracking systems: it avoids perspective distortion, distinguishes animals any color from any background and automatically evaluates vertical activity.ConclusionThe 3-D imaging system can be successfully applied for automated measurement of minipig behavior in neurobiological and psychopharmacological experiments.