کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6271309 | 1614756 | 2016 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
“Tasting” the cerebrospinal fluid: Another function of the choroid plexus?
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کلمات کلیدی
PBStransthyretinCPECTRPM5T1R1WGAPROPTTRPLCβ2IP3GPCRsPTCTBSICCDMEMFBSG protein-coupled receptors - G گیرنده های پروتئینی همراهinositol 1,4,5-trisphosphate - inositol 1،4،5-trisphosphateDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoImmunocytochemistry - ایمونوسیتوشیمیImmunofluorescence - ایمونوفلورسانسImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیTris-buffered saline - تریس بافر شورCNS - دستگاه عصبی مرکزیRoom temperature - دمای اتاقfetal bovine serum - سرم جنین گاوcentral nervous system - سیستم عصبی مرکزیblood–CSF barrier - مانع خون CSFPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریChoroid plexus - پیوند کوئیدWheat germ agglutinin - گیاه گندم آگلوتیینینBitter taste receptor - گیرنده طعم تلخ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The choroid plexus (CP) located in brain ventricles, by forming the interface between the blood and the cerebrospinal fluid (CSF) is in a privileged position to monitor the composition of these body fluids. Yet, the mechanisms involved in this surveillance system remain to be identified. The taste transduction pathway senses some types of molecules, thereby evaluating the chemical content of fluids, not only in the oral cavity but also in other tissues throughout the body, such as some cell types of the airways, the gastrointestinal tract, testis and skin. Therefore, we hypothesized that the taste transduction pathway could also be operating in the CP to assess the composition of the CSF. We found transcripts for some taste receptors (Tas1r1, Tas1r2, Tas1r3, Tas2r109 and Tas2r144) and for downstream signaling molecules (α-Gustducin, Plcβ2, ItpR3 and TrpM5) that encode this pathway, and confirmed the expression of the corresponding proteins in Wistar rat CP explants and in the CP epithelial cells (CPEC). The functionality of the T2R receptor expressed in CP cells was assessed by calcium imaging, of CPEC stimulated with the bitter compound d-Salicin, which elicited a rise in the intracellular Ca2+. This effect was diminished in the presence of the bitter receptor blocker Probenecid. In summary, we described the expression of the taste-related components involved in the transduction signaling cascade in CP. Taken together, our results suggest that the taste transduction pathway in CPEC makes use of T2R receptors in the chemical surveillance of the CSF composition, in particular to sense bitter noxious compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 320, 21 April 2016, Pages 160-171
Journal: Neuroscience - Volume 320, 21 April 2016, Pages 160-171
نویسندگان
J. Tomás, C.R.A. Santos, T. Quintela, I. Gonçalves,