کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6271946 | 1614776 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Orexins/hypocretins modulate the activity of NPY-positive and -negative neurons in the rat intergeniculate leaflet via OX1 and OX2 receptors
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کلمات کلیدی
TTXSCNPBSOXAmEPSCdLGNSB 334867mIPSCOrexin BOXBNDSNPYTCSBICOX1 receptortetrodotoxin citrateDMSO - DMSOElectrophysiology - الکتروفیزیولوژیEnk - انکenkephalin - انکافالینorexin A - اورکسین AOrexin - اورکسینIGL - ایگلvLGN - بیدارminiature inhibitory postsynaptic currents - جریان ممانعت کننده پسینپتیکminiature excitatory postsynaptic currents - جریانهای پسینپتیک هیجان انگیز مینیاتوریintergeniculate leaflet - جزوه بین المللیKYN - جنسیتDimethyl sulfoxide - دیمتیل سولفواکسیدBiological clock - ساعت زیستیnormal donkey serum - سرم عسل طبیعیGhT - غایتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریSuprachiasmatic nuclei - هسته های SuprachiasmaticPatch clamp - پچ گیرOrexin receptor 2 - گیرنده اورکسین 2orexin receptor 1 - گیرنده اکسین 1Neuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Orexins/hypocretins (OXA and OXB) are two hypothalamic peptides involved in the regulation of many physiological processes including the sleep-wake cycle, food intake and arousal. The orexinergic system of the lateral hypothalamus is considered a non-specific peptidergic system, and its nerve fibers innervate numerous brain areas. Among many targets of orexinergic neurons is the intergeniculate leaflet (IGL) of the thalamus - a small but important structure of the mammalian biological clock. In rats, the IGL consists of GABAergic cells which also synthesize different neuropeptides. One group of neurons produces neuropeptide Y (NPY) and sends its axons to the master biological clock known as the suprachiasmatic nuclei. Another neuronal group produces enkephalin and is known to connect contralateral IGLs. This study evaluated the effects of orexins on identified IGL neurons revealing that 58% of the recorded neurons were sensitive to OXA (200 nM) and OXB (200 nM) administration. Both NPY-positive and -negative neurons were depolarized by these neuropeptides. Experiments using selective orexin receptor antagonists (SB-334867, 10 μM and TCS-OX2-29, 10 μM) suggested that both orexin receptors participate in the recorded OXA effects. In addition, IGL neurons were either directly depolarized by OXA or their activity was altered by changes in presynaptic inputs. We observed an increase of GABA release onto the investigated IGL neuron after OXA application, consistent with a presynaptic localization of the orexin receptors. An increase in miniature excitatory postsynaptic current frequency was not observed within the IGL. Our findings reinforce the connection between circadian clock physiology and the orexinergic system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 300, 6 August 2015, Pages 370-380
Journal: Neuroscience - Volume 300, 6 August 2015, Pages 370-380
نویسندگان
K. Palus, L. Chrobok, M.H. Lewandowski,