کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6272389 1614772 2015 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities
ترجمه فارسی عنوان
ساختار توموگرافی الکترونی و ترکیب پروتئین مخچه موشهای جدا شده مغز، هیپوکامپ و پست سیناپتیک قشر
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- Electron tomography provides novel ultrastructural information of PSDs.
- PSDs from the cortex, hippocampus and cerebellum show distinct subsets of proteins.
- PSDs from cerebellum were composed of three distinct morphological classes.
- CaMKII is the most abundant protein in PSDs from each region.
- Proteasomes were more abundant in PSDs from the hippocampus and cerebellum than cortex.

Electron tomography and immunogold labeling were used to analyze similarities and differences in the morphology and protein composition of postsynaptic densities (PSDs) isolated from adult rat cerebella, hippocampi, and cortices. There were similarities in physical dimensions and gross morphology between cortical, hippocampal and most cerebellar PSDs, although the morphology among cerebellar PSDs could be categorized into three distinct groups. The majority of cerebellar PSDs were composed of dense regions of protein, similar to cortical and hippocampal PSDs, while others were either composed of granular or lattice-like protein regions. Significant differences were found in protein composition and organization across PSDs from the different brain regions. The signaling protein, βCaMKII, was found to be a major component of each PSD type and was more abundant than αCaMKII in both hippocampal and cerebellar PSDs. The scaffold molecule PSD-95, a major component of cortical PSDs, was found absent in a fraction of cerebellar PSDs and when present was clustered in its distribution. In contrast, immunogold labeling for the proteasome was significantly more abundant in cerebellar and hippocampal PSDs than cortical PSDs. Together, these results indicate that PSDs exhibit remarkable diversity in their composition and morphology, presumably as a reflection of the unique functional demands placed on different synapses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 304, 24 September 2015, Pages 286-301
نویسندگان
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