کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6272739 | 1614786 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thalamic adenylyl cyclase 1 is required for barrel formation in the somatosensory cortex
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کلمات کلیدی
thalamocorticalpKaPFACre/loxP system5-HTTPMBSFventrobasal nucleusNMDARAC1Refinement - اصلاحphosphate buffer - بافر فسفاتserotonin transporter - سروتونین حمل کنندهcytochrome oxidase - سیتوکروم اکسیدازSomatosensory system - سیستم Somatosensoryconditional knockout - شکستن شرطNeuronal circuit - مدار نورونMouse - موشparaformaldehyde - پارافرمالدهیدprotein kinase A - پروتئین کیناز AN-methyl-d-aspartate receptor - گیرنده N-methyl-d-aspartate
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cyclic AMP signaling is critical for activity-dependent refinement of neuronal circuits. Global disruption of adenylyl cyclase 1 (AC1), the major calcium/calmodulin-stimulated adenylyl cyclase in the brain, impairs formation of whisker-related discrete neural modules (the barrels) in cortical layer 4 in mice. Since AC1 is expressed both in the thalamus and the neocortex, the question of whether pre- or postsynaptic (or both) AC1 plays a role in barrel formation has emerged. Previously, we generated cortex-specific AC1 knockout (Cx-AC1KO) mice and found that these animals develop histologically normal barrels, suggesting a potentially more prominent role for thalamic AC1 in barrel formation. To determine this, we generated three new lines of mice: one in which AC1 is disrupted in nearly half of the thalamic ventrobasal nucleus cells in addition to the cortical excitatory neurons (Cx/pTh-AC1KO mouse), and another in which AC1 is disrupted in the thalamus but not in the cortex or brainstem nuclei of the somatosensory system (Th-AC1KO mouse). Cx/pTh-AC1KO mice show severe deficits in barrel formation. Th-AC1KO mice show even more severe disruption in barrel patterning. In these two lines, single thalamocortical (TC) axon labeling revealed a larger lateral extent of TC axons in layer 4 compared to controls. In the third line, all calcium-stimulated adenylyl cyclases (both AC1 and AC8) are deleted in cortical excitatory neurons. These mice have normal barrels. Taken together, these results indicate that thalamic AC1 plays a major role in patterning and refinement of the mouse TC circuitry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 290, 2 April 2015, Pages 518-529
Journal: Neuroscience - Volume 290, 2 April 2015, Pages 518-529
نویسندگان
A. Suzuki, L.-J. Lee, Y. Hayashi, L. Muglia, S. Itohara, R.S. Erzurumlu, T. Iwasato,