کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6273715 1614799 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activation of CB1 inhibits NGF-induced sensitization of TRPV1 in adult mouse afferent neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Activation of CB1 inhibits NGF-induced sensitization of TRPV1 in adult mouse afferent neurons
چکیده انگلیسی


- CB1, trkA, and TRPV1 are co-localized in a subpopulation of adult mouse afferent neurons.
- NGF increased the number of neurons that responded to capsaicin.
- CB1 agonist ACEA (10 nM) inhibited the NGF-induced responses.
- ACEA inhibited NGF-induced phosphorylation of AKT.
- Blocking PI3 kinase activity attenuated the NGF-induced responses.

Transient receptor potential vanilloid 1 (TRPV1)-containing afferent neurons convey nociceptive signals and play an essential role in pain sensation. Exposure to nerve growth factor (NGF) rapidly increases TRPV1 activity (sensitization). In the present study, we investigated whether treatment with the selective cannabinoid receptor 1 (CB1) agonist arachidonyl-2′-chloroethylamide (ACEA) affects NGF-induced sensitization of TRPV1 in adult mouse dorsal root ganglion (DRG) afferent neurons. We found that CB1, NGF receptor tyrosine kinase A (trkA), and TRPV1 are present in cultured adult mouse small- to medium-sized afferent neurons and treatment with NGF (100 ng/ml) for 30 min significantly increased the number of neurons that responded to capsaicin (as indicated by increased intracellular Ca2 + concentration). Pretreatment with the CB1 agonist ACEA (10 nM) inhibited the NGF-induced response, and this effect of ACEA was reversed by a selective CB1 antagonist. Further, pretreatment with ACEA inhibited NGF-induced phosphorylation of AKT. Blocking PI3 kinase activity also attenuated the NGF-induced increase in the number of neurons that responded to capsaicin. Our results indicate that the analgesic effect of CB1 activation may in part be due to inhibition of NGF-induced sensitization of TRPV1 and also that the effect of CB1 activation is at least partly mediated by attenuation of NGF-induced increased PI3 signaling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 277, 26 September 2014, Pages 679-689
نویسندگان
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