کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273765 | 1614798 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphorylation enhances recombinant HSP27 neuroprotection against focal cerebral ischemia in mice
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کلمات کلیدی
terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelingPBSFITCHSP27rCBF8-OHdGNeuNMCAO8-hydroxydeoxyguanosine - 8 هیدروکسی دیواکسی گوآزینBSA - BSAIba-1 - IBA-1bovine serum albumin - آلبومین سرم گاوmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیFocal ischemia - ایسکمی کانونیTUNEL - تونلRegional cerebral blood flow - جریان خون منطقه ای مغزیBlood–brain barrier - سد خونی مغزیBBB - سد خونی مغزیPhosphorylation - فسفریلاسیونfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتNeuroprotection - محافظت نورونی یا محافظت از عصبPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMAPKAP Kinase 2 - مکاپ کیناز 2Heat shock protein 27 - پروتئین شوک حرارت 27neuron-specific nuclear protein - پروتئین هسته ای اختصاصی نورون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Heat shock protein 27 (HSP27) exerts cytoprotection against many cellular insults including cerebral ischemia. We previously indicated that intravenous injection of HSP27 purified from human lymphocytes (hHSP27) significantly reduced infarct volume following cerebral ischemia-reperfusion injury, while recombinant HSP27 (rHSP27) was less effective. Phosphorylation is important for HSP27 function, and hHSP27 was more highly phosphorylated than rHSP27. We hypothesized that MAPKAP kinase 2 in vitro-phosphorylated rHSP27 (prHSP27) might increase its brain protection. Mice underwent transient 1-h middle cerebral artery occlusion (MCAO), and then received tail-vein injections of one of the following 1 h after reperfusion: hHSP27 as positive control, rHSP27, prHSP27, or bovine serum albumin (BSA) as control. We measured infarct volume, neurological deficits, neurological severity, physiological parameters, cell-death, oxidative stress, and inflammatory response. Compared with BSA controls (30.7 ± 3.1mm3, n = 5), infarct volume was reduced by 67% in the hHSP27 positive-control group (10.1 ± 4.6mm3, P < 0.001, n = 5), 17% following rHSP27 (25.4 ± 3.6mm3, P < 0.05, n = 5), and 46% following prHSP27 (16.5 ± 4.0mm3, P < 0.001, n = 9). Compared to the rHSP27 and BSA-treated groups, prHSP27 also reduced functional deficits, and significantly suppressed apoptosis, oxidative stress, and inflammatory responses. Here, we showed the superior neuroprotective effects of phosphorylated HSP27 by administering prHSP27. prHSP27 may be a useful therapeutic agent to protect against acute cerebral ischemic stroke.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 278, 10 October 2014, Pages 113-121
Journal: Neuroscience - Volume 278, 10 October 2014, Pages 113-121
نویسندگان
Y. Shimada, R. Tanaka, H. Shimura, K. Yamashiro, T. Urabe, N. Hattori,