کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6273886 | 1614807 | 2014 | 7 صفحه PDF | دانلود رایگان |
- Live-cell imaging was first used to study the effect of 2,5-HD on slow axonal transport in vitro.
- GFP-tagged protein and fluorescence microscope were used to explore NF-M transport.
- 2,5-HD impaired the levels of motor proteins associated with NF-M transport in DRG cells.
- 2,5-HD reduced ATP levels in DRG cells, which provides energy support for NF-M transport.
Chronic exposure to n-hexane induces peripheral-central axonopathy, mediated by its metabolite 2,5-hexanedione (2,5-HD), in occupational workers and experimental animals, but the underlying mechanism is still unclear. In the current study, we investigated the effects of 2,5-HD on middle-molecular-weight neurofilament (NF-M) axonal transport using live-cell imaging technique in cultured rat dorsal root ganglia (DRG) cells. PA-GFP-NF-M plasmid was transfected into DRG neurons and live-cell imaging was performed to observe the slow axonal transport of NF-M. The levels of cytoskeleton and motor proteins in DRG cells were detected by Western-blot and the concentration of ATP was determined using an ATP Assay Kit. The results showed that 2,5-HD administration resulted in a decrease of NF-M axonal transport and a reduction of three neurofilament subunits levels in DRG cells. Furthermore, 2,5-HD exposure significantly decreased ATP contents and the protein levels of kinesin heavy chain (KHC). These findings indicated that 2,5-HD reduced slow axonal transport, neurofilaments cargoes, motor proteins and ATP energy in rat DRG cells, which may contribute to 2,5-HD-induced neurotoxicity.
Journal: Neuroscience - Volume 269, 6 June 2014, Pages 192-198