Immortalization and functional characterization of rat arachnoid cell lines

▶This is the first report of an immortalized arachnoid cell line derived from normal primary cells. ▶We created immortalized rat arachnoid cell lines using two retroviral constructs. ▶Cell lines were characterized on the basis of phenotypic markers, growth properties, and physiology. ▶This paper has direct relevance to modeling hydrocephalus.