کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6276882 | 1295745 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of sigma receptor 1 mRNA and protein in rat retina
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کلمات کلیدی
DT-PBSACh - آهAcetylcholine - استیل کولینimmunofluorescence double labeling - برچسب ایمنی فلوئورسانسamacrine cell - سلول آماکریینbipolar cell - سلول دو قطبیconfocal laser scanning microscopy - میکروسکوپهای اسکن لیزری کانفوکالChAT - چتcalbindin - کلبیندینcholine acetyltransferase - کولین استیل ترانسفرازSigma receptor - گیرنده سیگما
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Sigma receptor (ÏR), known as a unique nonopiate, nonphencyclidine brain receptor, can bind diverse classes of psychotropic drugs, neurosteroids and other synthetic compounds, such as (+)pentazocine, etc. Two types of ÏRs have been identified: ÏR1 and ÏR2. In this work, we examined the expression of ÏR1 in rat retina by reverse transcription-polymerase chain reactive (RT-PCR) analysis and immunofluorescence double labeling. RT-PCR analysis showed that ÏR1 mRNA was present in rat retina. Furthermore, labeling for ÏR1 was diffusely distributed in the outer and inner plexiform layers. The ÏR1-immunoreactivity (IR) was also observed in many cells in the inner nuclear layer and the ganglion cell layer. In the outer retina ÏR1 was expressed in all horizontal cells labeled by calbindin. In contrast, no ÏR1-IR was detected in several subtypes of bipolar cells, including rod-dominant ON-type bipolar cells, types 2, 3, 5 and 8 bipolar cells, labeled by protein kinase C (PKC), recoverin and hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 (HCN4) respectively. In the inner retina, most of GABAergic amacrine cells, including dopaminergic and cholinergic ones, stained by tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) respectively, expressed ÏR1. Some glycinergic amacrine cells were also labeled by ÏR1, but glycinergic AII amacrine cells were not labeled. In addition, ÏR1-IR was seen in almost all somata of the ganglion cells retrogradely labeled by fluorogold. These results suggest that ÏR1 may have neuromodulatory and neuroprotective roles in the retina.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 167, Issue 4, 2 June 2010, Pages 1151-1159
Journal: Neuroscience - Volume 167, Issue 4, 2 June 2010, Pages 1151-1159
نویسندگان
L.L. Liu, L. Wang, Y.M. Zhong, X.L. Yang,