کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6276922 | 1295746 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Syntaxin 1A regulates dopamine transporter activity, phosphorylation and surface expression
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کلمات کلیدی
DATAMPHSNAREPKCphorbol 12-myristate 13-acetateSyntaxin 1APMA - LDC هاAmphetamine - آمفتامین هاPhorbol ester - افلبل استرDopamine transporter - انتقال دهنده دوپامینtyrosine hydroxylase - تیروزین هیدروکسیلازDopamine - دوپامینSynaptosomes - سیناپتوزوم هاTransport regulation - مقررات حمل و نقلProtein kinase C - پروتئین کیناز سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We investigated the functional relationship between the soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) protein syntaxin 1A (syn 1A) and the dopamine transporter (DAT) by treating rat striatal tissue with Botulinum Neurotoxin C (BoNT/C) and co-transfecting syn 1A with DAT in non-neuronal cells, followed by analysis of DAT activity, phosphorylation, and regulation. Treatment of striatal slices with BoNT/C resulted in elevated dopamine (DA) transport Vmax and reduced DAT phosphorylation, while heterologous co-expression of syn 1A led to reduction in DAT surface expression and transport Vmax. Syn 1A was present in DAT immunoprecipitation complexes, supporting a direct or indirect interaction between the proteins. Phorbol ester regulation of DA transport activity was retained in BoNT/C-treated synaptosomes and syn 1A transfected cells, demonstrating that protein kinase C (PKC) and syn 1A effects occur through independent processes. These findings reveal a novel mechanism for regulation of DAT activity and phosphorylation, and suggest the potential for syn 1A to impact DA neurotransmission through effects on reuptake.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 170, Issue 2, 13 October 2010, Pages 408-416
Journal: Neuroscience - Volume 170, Issue 2, 13 October 2010, Pages 408-416
نویسندگان
M.A. Cervinski, J.D. Foster, R.A. Vaughan,