کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277031 | 1295747 | 2010 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endocannabinoid-mediated synaptically evoked suppression of GABAergic transmission in the cerebellar cortex
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کلمات کلیدی
IPSPsspontaneous postsynaptic currentsPSCmGluR2-arachidonoylglycerolDNQXdepolarization-induced suppression of inhibitionDSIDSEaCSF6,7-dinitroquinoxaline-2,3-dione - 6،7-dinitroquinoxaline-2،3-dioneCB1 cannabinoid receptor - CB1 گیرنده کانابینوئیدDMSO - DMSOsIPSC - Şipscaendocannabinoid - آندوکانابینوئیدSpontaneous inhibitory postsynaptic current - جریان پستانیپتیک مهارکننده خود به خودیDimethylsulfoxide - دیمتیل سولفواکسیدdepolarization-induced suppression of excitation - سرکوب تحریک ناشی از دپولاریزاسیونRetrograde signaling - سیگنالینگ بازسازی شدهpre - قبل ازartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیinhibitory postsynaptic potentials - پتانسیل های پستانیپتیک مهارکنندهSynaptic plasticity - پلاستیسیته سیناپسیvoltage-gated calcium channels - کانال های کلسیم با ولتاژMetabotropic glutamate receptor - گیرنده گلوتامات متابوتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Presynaptic CB1 cannabinoid receptors are frequently targets of endogenous cannabinoids (endocannabinoids) released from postsynaptic neurons. It is known that the glutamatergic afferent input to a neuron can trigger endocannabinoid production and that the released endocannabinoid can suppress the glutamatergic input. We tested the hypothesis that activation of the glutamatergic input to a neuron leads to an endocannabinoid-mediated suppression of the GABAergic afferent input to the same neuron. Spontaneous postsynaptic currents (sPSCs) were recorded with patch-clamp techniques in Purkinje cells in mouse cerebellar brain slices. Activation of the climbing fiber-mediated glutamatergic input to Purkinje cells led to a suppression of the sPSCs by 34±3%. This suppression was mostly due to suppression of GABAergic spontaneous inhibitory postsynaptic current (sIPSCs), because 93% of the sPSCs recorded in Purkinje cells were GABAergic sIPSCs. Blockade of ionotropic, but not metabotropic glutamate receptors, prevented the suppression. The climbing fiber activation led to an increase in calcium concentration in the Purkinje cells, and this increase was necessary for the suppression of sPSCs, because the suppression did not occur when the calcium increase was prevented by BAPTA. No sPSC suppression was observed in the presence of the CB1 antagonist rimonabant or the diacylglycerol lipase inhibitor orlistat. In a further series of experiments GABAergic sIPSCs were recorded: these sIPSCs were also suppressed after climbing fiber activation, and the suppression was sensitive to the CB1 antagonist SLV319. Finally, the GABAergic synaptic transmission between molecular layer interneurons and Purkinje cells was directly studied on simultaneously patch-clamped neuron pairs. Climbing fiber activation led to suppression of the interneuron â Purkinje cell synaptic transmission. The results point to a novel form of endocannabinoid-mediated heterosynaptic plasticity. The endocannabinoid production in a neuron is triggered by its glutamatergic synaptic input and is dependent on an increase in intracellular calcium concentration. The produced endocannabinoid, in turn, suppresses the GABAergic synaptic input to the neuron by activating CB1 cannabinoid receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 169, Issue 3, 1 September 2010, Pages 1268-1278
Journal: Neuroscience - Volume 169, Issue 3, 1 September 2010, Pages 1268-1278
نویسندگان
M.J. Urbanski, F.E. Kovacs, B. Szabo,