کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277259 | 1295749 | 2010 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preservation of transendothelial glucose transporter 1 and P-glycoprotein transporters in a cortical slice culture model of the blood-brain barrier
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کلمات کلیدی
GLUT-1R123P-gpblocking solutionTBSTFGF-2NeuNABCPBSMDRGFAPP-glycoprotein - P-گلیکوپروتئینDIV - دیوRhodamine 123 - رودامین 123day in vitro - روز in vitropostnatal day - روز پس از زایمانBBB - سد خونی مغزیBlood–brain barrier - سد خونی مغزیEndothelial cells - سلولهای اندوتلیالBrain vasculature - عروق مغزPhosphate buffered saline - فسفات بافر شورMouse - موشGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالMultidrug resistance gene - ژن مقاومت چندین رژیمATP-binding cassette - کیت اتصال به ATPglucose transporter 1 - گلوکز 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preservation of transendothelial glucose transporter 1 and P-glycoprotein transporters in a cortical slice culture model of the blood-brain barrier Preservation of transendothelial glucose transporter 1 and P-glycoprotein transporters in a cortical slice culture model of the blood-brain barrier](/preview/png/6277259.png)
چکیده انگلیسی
A variety of neurological diseases are characterized by disturbances of the blood-brain barrier (BBB) and its transporters. We recently introduced fibroblast growth factor treated cortical organotypic slice cultures of mice as a model for in vitro studies of the blood-brain barrier and have now further characterized the maintenance and function of transport-proteins typically expressed in the endothelium of cerebral blood vessels. The glucose transporter GLUT-1 is present in blood vessels of slice cultures derived from postnatal day 4 to 21 mice after 3 days in vitro. The endothelial multidrug resistance P-glycoprotein (P-gp) which is involved in the control of pharmacological substance transport across the blood-brain barrier is also maintained in blood vessels, most prominently in slice cultures derived from postnatal day 14 and 21 mice. To assess P-gp function, we tested rhodamine 123 transport in presence or absence of the P-gp inhibitor verapamil. Rhodamine 123-fluorescence accumulated rapidly in the vascular lumen both in acute slices and in slices cultured for 3 days in vitro. Our results provide evidence that endothelial transporters and their functional properties can be maintained in organotypic cortical slices cultures, thus making it an attractive model system for the study of the blood-brain barrier.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 170, Issue 1, 29 September 2010, Pages 361-371
Journal: Neuroscience - Volume 170, Issue 1, 29 September 2010, Pages 361-371
نویسندگان
R.S. Camenzind, S. Chip, H. Gutmann, J.P. Kapfhammer, C. Nitsch, K. Bendfeldt,