کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
627785 1455471 2008 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The study of continuous membrane crystallization on lysozyme
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
The study of continuous membrane crystallization on lysozyme
چکیده انگلیسی

Membrane crystallization, as a new separation process, was developed in the past few years. The new crystallization technique claims to be capable of producing perfect crystals under suitable operating conditions. In this study, membrane crystallization on lysozyme was discussed in this work. The influence of various membrane crystallization conditions on the crystal habit and quality of lysozyme was studied, and these conditions include protein concentration, precipitant concentration, the flow velocity of crystallization solutions, the flow velocity and concentration of stripping solutions, the pH and also ion strength of crystallization solutions. The changes of crystallization solutions concentration with time and the induction time were also studied. Furthermore, crystallization conditions were optimized, and the IR spectra from crystals obtained were made. Perfect lysozyme crystals were obtained. Two different crystal forms have been obtained, depending upon protein concentration, pH and precipitating salt employed. Compared with the traditional crystallization methods, membrane crystallization is more rapid and with slower initial protein concentration. In addition, membrane crystallization can control the crystallization process better, and better crystals were obtained.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Desalination - Volume 219, Issues 1–3, 25 January 2008, Pages 101-117