کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278179 | 1295800 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vasoactive intestinal peptide in rats with focal cerebral ischemia enhances angiogenesis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
VIPDABPBSHIFDAPIMCAECAFBSHBSSMCAO4′,6′-diamidino-2-phenylindole - 4 '، 6'-diamidino-2-phenylindoleMTT - MTTAngiogenesis - آنژیوژنزmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیCerebral ischemia - ایسکمی مغزیBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاdiaminobenzidine - دیامینو بنزیدینfetal bovine serum - سرم جنین گاوRat brain microvascular endothelial cells - سلول های اندوتلیال مغز استخوان مغز موش صحراییmiddle cerebral artery - شریان مغزی میانیexternal carotid artery - شریان کاروتید خارجیHypoxia-inducible factor - فاکتور القاء کننده هیپوکسیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHanks' balanced salt solution - محلول نمک متعادل هانکسRats - موش صحراییvasoactive intestinal peptide - پپتید روده روده ای
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We studied the effect of vasoactive intestinal peptide (VIP) on angiogenesis in the ischemic boundary area after focal cerebral ischemia. Adult male Sprague-Dawley rats underwent middle cerebral artery occlusion for 2 h. A single dose of VIP was given via i.c.v. injection at the beginning of reperfusion. Immunohistochemistry and Western blotting were performed to assay angiogenesis and brain levels of vascular endothelial growth factor (VEGF) protein, respectively. In addition, the expression of VEGF and its receptors (flt-1 and flk-1), as well as endothelial proliferation, was measured using rat brain microvascular endothelial cells. Immunohistochemical analyses revealed significant (P<0.05) increases in the numbers of bromodeoxyuridine (BrdU) positive endothelial cells and microvessels at the boundary of the ischemic lesion in rats treated with VIP compared with rats treated with saline. Western blotting analysis showed that treatment with VIP significantly (P<0.05) raised VEGF levels in the ischemic hemisphere. In addition, treatment with VIP increased flt-1 and flk-1 immunoreactivity in endothelial cells. In vitro, incubation with VIP significantly (P<0.01) increased the proliferation of endothelial cells and induced the expression of VEGF, flt-1 and flk-1 in endothelial cells. The stimulatory effect of VIP on the proliferation of endothelial cells was significantly (P<0.01) inhibited by SU5416, a selective inhibitor of VEGF receptor tyrosine kinase. Our data suggest that treatment with VIP enhances angiogenesis in the ischemic brain, and this effect may be mediated by increases in levels of VEGF and its receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 161, Issue 2, 30 June 2009, Pages 413-421
Journal: Neuroscience - Volume 161, Issue 2, 30 June 2009, Pages 413-421
نویسندگان
J. Yang, C.H. Zong, Z.H. Zhao, X.D. Hu, Q.D. Shi, X.L. Xiao, Y. Liu,