کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278778 | 1295897 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adenosine inhibits basal forebrain cholinergic and noncholinergic neurons in vitro
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کلمات کلیدی
LDTinwardly rectifying potassium currentiKIRIndocarbocyanineIADVLPOIR-DICCy3REMTTX4-APPBSaCSFCPTIC504-aminopyridine - 4-آمینوپیریدینcyclic AMP - AMP cycliccAMP - cAMPDMSO - DMSOV1/2 - V1 / 2Adenosine - آدنوزینelectroencephalographic - الکتروانسفالوگرافیafterhyperpolarization - بعد از پمپاژtetrodotoxin - تترو دوتوکسین hyperpolarization-activated cation current - جریان کاتیونی فعال hyperpolarization فعال استrapid eye movement sleep - حرکت چشم سریع خوابSleep - خوابDimethyl sulfoxide - دیمتیل سولفواکسیدLaterodorsal tegmental - زایمان زودرسAHP - فرایند تحلیل سلسلهمراتبیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریEEG - نوار مغزیVentrolateral preoptic nucleus - هسته preoptic VentrolateralMembrane potential - پتانسیل غشاء basal forebrain - پیشانی پایهChAT - چتinfrared differential interference contrast - کنتراست تداخل اختلاط مادون قرمزcholine acetyltransferase - کولین استیل ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adenosine has been proposed as a homeostatic “sleep factor” that promotes the transition from waking to sleep by affecting several sleep-wake regulatory systems. In the basal forebrain, adenosine accumulates during wakefulness and, when locally applied, suppresses neuronal activity and promotes sleep. However, the neuronal phenotype mediating these effects is unknown. We used whole-cell patch-clamp recordings in in vitro rat brain slices to investigate the effect of adenosine on identified cholinergic and noncholinergic neurons of the magnocellular preoptic nucleus and substantia innominata. Adenosine (0.5-100 μM) reduced the magnocellular preoptic nucleus and substantia innominata cholinergic neuronal firing rate by activating an inwardly rectifying potassium current that reversed at â82 mV and was blocked by barium (100 μM). Application of the A1 receptor antagonist 8-cyclo-pentyl-theophylline (200 nM) blocked the effects of adenosine. Adenosine was also tested on two groups of electrophysiologically distinct noncholinergic magnocellular preoptic nucleus and substantia innominata neurons. In the first group adenosine, via activation of postsynaptic A1 receptors, reduced spontaneous firing via inhibition of the hyperpolarization-activated cation current. Blocking the H-current with ZD7288 (20 μM) abolished adenosine effects on these neurons. The second group was not affected by adenosine. These results demonstrate that, in the magnocellular preoptic nucleus and substantia innominata region of the basal forebrain, adenosine inhibits both cholinergic neurons and a subset of noncholinergic neurons. Both of these effects occur via postsynaptic A1 receptors, but are mediated downstream by two separate mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 140, Issue 2, 2006, Pages 403-413
Journal: Neuroscience - Volume 140, Issue 2, 2006, Pages 403-413
نویسندگان
E. Arrigoni, N.L. Chamberlin, C.B. Saper, R.W. McCarley,