کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6279336 | 1615071 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calcitonin gene-related peptide increases acetylcholine quantal size in neuromuscular junctions of mice
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کلمات کلیدی
EPPpKaCGRPNMJhCGRPVesamicolRMPmEPPACh - آهAChE - آهیNeuromuscular junction - اتصال عصبی عضلانیAcetylcholine - استیل کولینAcetylcholinesterase - استیل کولین استرازQuantal size - اندازه کوانتالminiature endplate potential - پتانسیل اندیکاتور مینیاتوریresting membrane potential - پتانسیل غشای استراحتendplate potential - پتانسیل پایان دادن بهprotein kinase A - پروتئین کیناز Acalcitonin gene-related peptide - پپتید مرتبط با ژن کلسی تونین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We used an intracellular microelectrode technique to study the mechanisms of action of two isoforms (human and rat) of calcitonin gene-related peptide (CGRP) on the evoked and spontaneous quantal secretion of acetylcholine (ACh) in mouse diaphragm motor synapses. Recordings of miniature endplate potentials (MEPPs) and evoked multiquantal endplate potentials (EPPs) in a cut neuromuscular preparation showed that CGRP increased the amplitude of EPPs without influencing their quantal content. Both isoforms of CGRP in a wide range of concentrations (1 nM-1 μM) provoked a similar considerable increase in MEPPs amplitude in a dose-dependent manner (up to 150-160% compared to control) without changing their frequency, rise-time, and decay. Inhibition of CGRP-receptors by truncated CGRP (CGRP8-37) completely prevented the potentiating effect of CGRP on the MEPPs amplitude. The effect of CGRP was not accompanied by changes in input resistance of muscle fiber membrane but was fully prevented by inhibition of vesicular ACh transport by vesamicol. Inhibition of protein kinase A (PKA) by H-89 also prevented CGRP action on the MEPPs amplitude. It is concluded that, in mammalian neuromuscular junctions, different isoforms of exogenously applied CGRP uniformly potentiate amplitudes of evoked and spontaneous postsynaptic potentials acting presynaptically via an increase in ACh quantal size.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 628, 15 August 2016, Pages 17-23
Journal: Neuroscience Letters - Volume 628, 15 August 2016, Pages 17-23
نویسندگان
Alexander E. Gaydukov, Polina O. Bogacheva, Olga P. Balezina,