Research paperAbnormally increased surface expression of AMPA receptors in the cerebellum, cortex and striatum of Cln3−/− mice

- We examined AMPA receptor surface expression in a mouse model of CLN3 disease.
- Surface levels of GluA1 and GluA2 were elevated in the cerebellum of Cln3−/− mice.
- Surface level of GluA2 was elevated in the cortex and striatum of Cln3−/− mice.
- These data suggest that CLN3 is involved in AMPA receptor cycling.

Mutations in the CLN3 gene cause a fatal neurodegenerative disorder, juvenile CLN3 disease. Exploring the cause of the motor coordination deficit in the Cln3−/− mouse model of the disease we have previously found that attenuation of AMPA receptor activity in 1-month-old Cln3−/− mice significantly improves their motor coordination [20]. To elucidate the mechanism of the abnormally increased AMPA receptor function in Cln3−/− mice, we examined the surface expression of AMPA receptors using surface cross-linking in brain slices from 1-month-old wild type (WT) and Cln3−/− mice. In surface cross-linked brain samples, Western blotting for AMPA receptor subunits revealed significantly increased surface levels of GluA1 and GluA2 in the cerebellum, and of GluA2 in the cortex and striatum of Cln3−/− mice as compared to WT mice. Expression levels of the GluA4 subunit were similar in the cerebellum of WT and Cln3−/− mice. While intracellular GluA1 levels in the WT and Cln3−/− cerebellum or cortex were similar, the intracellular expression of GluA1 in the Cln3−/− striatum was decreased to 56% of the WT level.Our results show a prominent increase in AMPA receptor surface expression in the brain of Cln3−/− mice and suggest that CLN3 is involved in the regulation of AMPA receptor surface expression.