کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6280661 | 1615096 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gintonin stimulates gliotransmitter release in cortical primary astrocytes
ترجمه فارسی عنوان
گینتونین انتشار گلیوترانسمیتر را در آستروسیت های اولیه قشر تحریک می کند
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کلمات کلیدی
FBSHEPESLPAGLP[Ca2+]i transientsN-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid[Ca2+]i - [Ca2 +] iAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPAstrocytes - آستروسیتlysophosphatidic acid - اسید لیسفسفیدیدGinseng - جینسنگfetal bovine serum - سرم جنین گاوintracellular calcium concentration - غلظت کلسیم داخل سلولیlysophosphatidic acid receptor - گیرنده اسید لیسوفسفیدیدGintonin - گینتونین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Lysophosphatidic acid (LPA) is a simple and minor phospholipid, but serves as a lipid-derived neurotransmitter via activation of G protein-coupled LPA receptors. Astrocytes abundantly express LPA receptors and contain gliotransmitters that modulate astrocyte-neuron interactions. Gintonin is a novel ginseng-derived G protein-coupled LPA receptor ligand. Gintonin induces [Ca2+]i transients in neuronal and non-neuronal cells via activation of LPA receptors, which regulate calcium-dependent ion channels and receptors. A line of evidence shows that neurotransmitter-mediated [Ca2+]i elevations in astrocytes are coupled with gliotransmitter release. However, little is known about whether gintonin-mediated [Ca2+]i transients are coupled to gliotransmitter release in astrocytes. In the present study, we examined the effects of gintonin on adenosine triphosphate (ATP) and glutamate release in mouse cortical primary astrocytes. Application of gintonin to astrocytes induced [Ca2+]i transients in a concentration-dependent and reversible manner. However, ginsenosides, other active ingredients in ginseng, had no effect on [Ca2+]i transients. The induction of gintonin-mediated [Ca2+]i transients was attenuated/blocked by the LPA1/3 receptor antagonist Ki16425, a phospholipase C inhibitor, an inositol 1,4,5-triphosphate receptor antagonist, and an intracellular Ca2+ chelator. Gintonin treatment on astrocytes increased ATP and glutamate release in a concentration- and time-dependent manner. BAPTA and Ki16425 attenuated gintonin-mediated ATP and glutamate release in astrocytes. The present study shows that gintonin-mediated [Ca2+]i transients are coupled to gliotransmitter release via LPA receptor activation. Finally, gintonin-mediated [Ca2+]i transients and gliotransmitter release from astrocytes via LPA receptor activation might explain one mechanism of gintonin-mediated neuromodulation in the central nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 603, 31 August 2015, Pages 19-24
Journal: Neuroscience Letters - Volume 603, 31 August 2015, Pages 19-24
نویسندگان
Hyunsook Kim, Byung-Hwan Lee, Sun-Hye Choi, Hyeon-Joong Kim, Suk-Won Jung, Sung-Hee Hwang, Hyewon Rhim, Hyung-Chun Kim, Ik-Hyun Cho, Seung-Yeol Nah,