کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6280744 1615102 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Insulin resistance due to dietary iron overload disrupts inner hair cell ribbon synapse plasticity in male mice
ترجمه فارسی عنوان
مقاومت انسولین به علت اضافه بار در رژیم غذایی باعث آسیب رسیدن به سلول موی سیناپس در موشهای نر می شود
کلمات کلیدی
بیش از حد آهن، مقاومت به انسولین، سلول های داخلی داخلی، سیناپس ریل،
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی
To evaluate whether cochlear inner hair cells (IHCs) ribbon synapse plasticity would be interrupted by insulin resistance (IR) due to dietary iron overload, we established an IR model in C57Bl/6 male mice with an iron-enriched diet for 16 weeks. Glucose levels were measured at weeks 4, 8, 12, 16. Glucose tolerance test and insulin tolerance test were performed at week 16 after overnight fasting. Then, auditory brainstem responses (ABRs) measurements were performed for hearing threshold shifts. After ABR measurements, cochleae were harvested for assessment of the number of IHC ribbon synapses by immunostaining, the morphology of cochlear hair cells and spiral ganglion neurons (SGNs) by transmission electron microscopy or immunostaining. Here, we show that IR due to dietary iron overload decreased the number of ribbon synapses, and induced moderate ABR threshold elevations. Besides, additional components including outer hair cells (OHCs), IHCs, and SGNs were unaffected. Moreover, IR did not disrupt the expression of vesicular glutamate transporter 3 (VGLUT3), myosin VIIa and prestin in hair cells. These results indicate that IHC ribbon synapses may be more susceptible to IR due to dietary iron overload.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 597, 15 June 2015, Pages 183-188
نویسندگان
, , , , , , , ,