Possible effects of nitric oxide synthases on odor-induced behavioral changes in mice

Although exposure to olfactory stimulants can induce neurobehavioral changes, there is a lack of data regarding specific effects on neurotransduction, especially nitric oxide (NO)-mediated neurotransduction. We investigated the relationship between altered behavioral performance and changes in the expression of nitric oxide synthase (NOS), nNOS, iNOS, and eNOS, in 2-methylbutyric (2MB) acid-exposed mice. Mice were exposed to 2MB acid by inhalation and olfactory preference/avoidance and motor coordination were measured. Additionally, we examined NOS expression in the olfactory bulb of the mouse brain. Mice exposed to 2MB acid showed significant changes in olfactory preference and rotarod activity as compared with controls. Although there was no change in nNOS and iNOS expressions in the olfactory bulb of 2MB acid-exposed mice, eNOS expression increased significantly in the olfactory bulb of 9.0 M 2MB acid-exposed mice. These data indicate that altered eNOS expression in the olfactory bulb may contribute to 2MB acid-induced behavioral changes.