کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282467 | 1615141 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and characterization of the ionotropic GABA receptor subunit Ï1 from pig (Sus scrofa)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Since human and pig eyes have remarkably anatomical and physiological similitudes swine models have been broadly used for functional studies and therapeutic research. Recently, a GABAÏ-mediated relaxation of retinal vascularity suggested that GABAÏ signaling may be used to improve retinal blood flow in vascular-driven impaired vision, and a further molecular characterization of GABAÏ receptors would be beneficial. However, none of the GABAÏ type subunits from pigs has been yet cloned; Among the 19 subunits that compose the family of GABAA receptors, Ï1-3 subunits are capable of forming homomeric channels. These homomeric receptors are particularly interesting because their pharmacological and kinetic properties are notably different from receptors composed by other GABAA subunits. Here we report the cloning of the GABAÏ1subunit from the pig and the functional expression of homomeric channels in Xenopus oocytes. The most notable difference found in the pig GABAÏ1 receptor was the absence of a stretch of 17 amino acids near the amino terminus (R41-V58) conserved in the rat and the human. This sequence has a higher nucleotidic match with the transcript variant 2 of the human GABAÏ1 subunit. Xenopus oocytes injected with cRNA from the receptor generated currents when exposed to GABA that shared all the characteristics of other GABAÏ1 subunits in mammals, including its modulation by dopamine. This study will help to increase the knowledge of the genetics of the pig, further the understanding of this important neurotransmitter receptor family and will shed some light in the evolution of these genes among mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 78-81
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 78-81
نویسندگان
Jorge Mauricio Reyes-Ruiz, Agenor Limon, Ricardo Miledi,