کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282501 | 1615141 | 2014 | 5 صفحه PDF | دانلود رایگان |
- We studied kinetic aspects of oxygen/glucose deprivation (OGD) in hippocampal slices.
- OGD led to a reduction in the population spike amplitude.
- Preconditioning requires a time window of ca. 60Â min.
Preconditioning increases the neurons' resistance to subsequent hypoxia. An in vitro study was conducted to explore kinetic aspects of hypoxic preconditioning. Hippocampal slices were exposed to one single or repeated episodes of oxygen and glucose deprivation (OGD). The interval between OGD episodes varied between 30Â min and 180Â min. OGD led to a significant reduction in the population spike amplitude. Subsequent episodes of OGD did not result in a further reduction in the population spike amplitude if the interval between the episodes was ca. 60Â min, which demonstrated that there were preconditioning effects. In the experiment using an interval of 30Â min, population spike amplitude decreased after each OGD episode. The set-up described is useful for detecting damaging effects of OGD as well as preconditioning effects. A time window of ca. 60Â min is required to induce protective mechanisms.
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 175-179