کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6282737 1615147 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Paraventricular nucleus nesfatin-1 neurons are regulated by pituitary adenylate cyclase-activating polypeptide (PACAP)
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Paraventricular nucleus nesfatin-1 neurons are regulated by pituitary adenylate cyclase-activating polypeptide (PACAP)
چکیده انگلیسی
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide also related to the stress response, feeding, and regulation of cardiovascular and autonomic nervous systems. The neurons with receptors for PACAP are distributed in PVN. However, there are no reports showing the direct effect of PACAP on nesfatin-1 neurons. In order to explore the direct effect of PACAP on PVN nesfatin-1 neuron, we have measured the cytosolic free calcium ([Ca2+]i) using fura-2 microfluorometry in single neurons isolated from PVN of adult rats, followed by immunocytochemical identification of nesfatin-1 neurons. PACAP at 10−15 M to 10−9 M increased [Ca2+]i in dose dependent manner. PAC1 and VPAC2 receptor agonists also increased [Ca2+]i. Sixteen out of 40 neurons (40%) in PVN responded to 10−9 M PACAP, and 12 out of 16 neurons (75%) which responded to 10−9 M PACAP were found to be nesfatin-1 neurons. In this paper we show that PACAP directly activates nesfatin-1 neurons in PVN. The data suggest that nesfatin-1 controls feeding, stress response or autonomic response under PACAP regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 551, 13 September 2013, Pages 39-42
نویسندگان
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