کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6283033 | 1615150 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed](/preview/png/6283033.png)
چکیده انگلیسی
Specific neuron ablation with laser microbeam has been used in behavioral analysis of Caenorhabditis elegans. However, this method is hard to acquire many ablated worms, and is unable to compare behavioral changes just before and after ablation. Here, we developed an ablation method by using genetically encoded photosensitizer protein, KillerRed, which produces reactive oxygen species by green light irradiation. Ablation of AWA sensory neurons abolished the chemotaxis to AWA specific sensitive attractant, diacetyl, and no functional effect on the other sensory neuron, AWC, which senses benzaldehyde. This ablation method can be useful for analyzing neural in situ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 548, 26 August 2013, Pages 261-264
Journal: Neuroscience Letters - Volume 548, 26 August 2013, Pages 261-264
نویسندگان
Junya Kobayashi, Hisashi Shidara, Yuma Morisawa, Maki Kawakami, Yuta Tanahashi, Kohji Hotta, Kotaro Oka,