Biochemical, single-channel, whole-cell patch clamp, and pharmacological analyses of endogenous TRPM4 channels in HEK293 cells

► HEK293 cells express endogenous TRPM4 channels. ► We performed a biochemical and biophysical analysis of TRPM4 channels. ► Biophysical properties of endogenous TRPM4 channels are similar to transfected ones.