Calretinin is expressed in the intermediate cells during olfactory receptor neuron development

• Calretinin was expressed at the stage between those of beta-III tubulin and OMP expressions.
• Calretinin was co-expressed with beta-III tubulin or OMP, or was expressed exclusively.
• Calretinin may serve as a developing ORN marker presenting an intermediate stage of development.

The field of neurogenesis has greatly benefited from stage-specific marker discoveries. However, such markers are not well defined in the olfactory epithelium (OE), where olfactory receptor neurons (ORNs) are constantly generated throughout lifetime. During OE neurogenesis, there is a lack of markers that label cells that are at the intermediate stage before they are fully mature. In this study, we show that during embryonic development calretinin is expressed transiently in the intermediate cells right before ORNs become mature. Calretinin is expressed between the end of beta-III tubulin (an immature neuronal marker) expression and the beginning of olfactory marker protein (OMP, a mature neuronal marker) expression in ORNs. Therefore, calretinin can serve as a marker of the intermediate ORNs. With this discovery, future studies can use calretinin as a tool to define these intermediate ORNs during olfactory neurogenesis.