Immunohistochemical analysis of the colocalization of corticotropin-releasing hormone receptor and glucocorticoid receptor in kisspeptin neurons in the hypothalamus of female rats

► Almost all kisspeptin neurons in both of AVPV/PeN and ARC expressed CRH-R. ► A few close appositions of CRH fibers on some of kisspeptin neurons were observed. ► Many kisspeptin neurons in AVPV/PeN expressed GR. ► On the other hand, only a few kisspeptin neurons in ARC expressed GR.