Immunoreactivity of the amino-terminal portion of the amyloid-beta precursor protein in the nucleolus

The functioning and metabolic pathway of the amyloid β-precursor protein (APP) have not been fully elucidated. To fill this research gap, this study immunocytochemically investigated the intracellular localization of APP in the neuroblastoma cell line SK-N-SH and in normal primary cells. Using antibodies against the amino-terminal portion of the APP molecule, immunoreactivity was detected not only in the cytoplasm but also in the nucleus and nucleolus. Further analysis revealed the co-localization of amino acids 44-63 of the APP molecule with fibrillarin, a nucleolus marker. These findings indicate that a fraction of APP, including its amino-terminal portion, may be localized in the nucleus as well as in the nucleolus, suggesting an important role of APP in RNA metabolism and other intra-nucleolus functions.

► The N-terminal portion of APP localizes in the nucleus and the nucleolus. ► The GS4463 antibody co-localizes with APP and fibrillarin, the nucleolus marker. ► These findings indicate the function of APP in the nucleolus.