Optineurin in Huntington's disease intranuclear inclusions

Optineurin mutations cause adult-onset primary open-angle glaucoma and have been associated with some familial forms of amyotrophic lateral sclerosis (ALS). Optineurin is involved in many cellular processes and interacts with a variety of proteins, among them huntingtin (htt). Here we report that in Huntington's disease (HD) cortex, optineurin frequently occurs in neuronal intranuclear inclusions, and to a lesser extent, in inclusions in the neuropil and in perikarya. Most intranuclear optineurin-positive inclusions were co-labeled for ubiquitin, but they were only occasionally and more weakly co-labeled for htt. Optineurin-labeled neuropil and perikaryal inclusions were commonly co-labeled for ubiquitin and htt. Although these inclusions were common in cortex, they were rare in striatum. Our results show that in HD optineurin is present in intranuclear, neuropil and perikaryal inclusions. It is not clear whether this indicates a primary involvement in the disease process. In HD, the known interaction of htt and optineurin may suggest that a different process takes place as compared to other neurodegenerative disorders.

► It has been reported that optineurin mutations cause forms of glaucoma. ► More recently it was shown that it is found in inclusions in neurodegenerative disorders. ► We report that in Huntington's disease, optineurin often occurs in intranuclear inclusions. ► It can also be detected in inclusions in the neuropil and perikarya. ► In these inclusions Optineurin is often colocalized with ubiquitin and huntingtin.