Dual immunofluorescence study of citrullinated proteins in Parkinson diseased substantia nigra

► In control substantia nigra (SN), citrullinated proteins are only in astrocytes. ► In Parkinson diseased (PD) SN, citrullinated proteins are also in neurons. ► Citrullinated proteins in PD SN dopamine neurons are located in the cytoplasm. ► Citrullinated proteins in PD SN neurons are not restricted to Lewy bodies.